Anatomy & Physiology of Molecules and Substance Questions Please if you are a specialist in Anatomy and Physiology, answer the questions in the attached document. Thanks! Lab 2: Solutions and Cellular Transport
Name: ___________________________
Answer the following questions and turn in your answers (via Canvas) for this week’s lab grade.
Lab 1a. Solutions and Colligative Properties
A. Calculate how much of each of the following reagents are needed to prepare 100 ml of the following four
solutions and report your result in the first column of the table below (2pts).
1.
2.
3.
4.
2 M glucose
25% glucose
1 M CaCl2
10% CaCl2
B. Predict how the conductivity and boiling point of these four solutions changes relative to deionized
water and report your results below (increase, decrease, or no change). (3pts)
Amount in
grams
CONDUCTIVITY
BOILING POINT
in Celsius
2 M glucose
25%
glucose
1 M CaCl2
10%
CaCl2
DI H2O
Poor
Briefly explain your predictions for both reagents in the space given below:
Glucose:
Calcium Chloride:
100 C
Lab 2a Questions about PhysioEx activities
ACTIVITY 1: Simulating Dialysis (Simple diffusion)
1. Describe two variables that affect the rate of diffusion.
2. Why is the urea not able to diffuse through the 20 MWCO membrane? Why is the glucose able to
diffuse through 200 MWCO membrane but not albumin?
3. Put the following in order from smallest to largest molecular weight: glucose, albumin, sodium
chloride, and urea
ACTIVITY 2: Simulated Facilitated Diffusion
1. Explain one way in which facilitated diffusion is the same a simple diffusion and one way in which it
differs.
2. The larger value obtained when more glucose carriers were present corresponds to an increase in
the rate of glucose transport. Explain why the rate increased.
3. Describe the effect that Na+Cl- had on glucose transport and provide an explanation for the obtained
result.
ACTIVITY 3: Simulated Osmotic Pressure
1. Explain the effect that increasing the Na+Cl- concentration had on osmotic pressure and why it has
this effect.
2. Describe one way in which osmosis is similar to simple diffusion and one way in which it is different.
3.
Explain the results when the conditions were 9 mM albumin in the left beaker and 10 mM glucose
in the right beaker with the 200 MWCO membrane in place
ACTIVITY 4: Simulated Filtration
1. Explain in your own words why increasing the pore size increased the filtration rate. Use an analogy
to support your statement.
2. Which solute did not appear in the filtrate using any of the membranes? Explain why.
3. Why did increasing the pressure increase the filtration rate but not the concentration of solutes?
ACTIVITY 5: Simulating Active Transport
1. Describe the significance of using 9 mM Na+Cl- inside the cell and 6 mM K+Cl- outside the cell, instead
of other concentration ratios.
2. Explain why there was no sodium transport when potassium was absent even though ATP was
present.
3. Explain why the addition of glucose carriers had no effect on sodium and potassium transport. What
is the mode of transport of glucose in this experiment (active or passive; simple or facilitated)?
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